Volume No. 7 Issue No.: 4A Page No.: 1496-1500 April-June 2013




Syed Shabudeen P.S.*, Soundrarajan M. and Indumathi P.

Department of Chemistry and Environmental Science, Kumaraguru College of Technology,
Coimbatore (INDIA)


Received on : November 07, 2012




The inorganic molecules namely carbon dioxide and water using energy from solar radiation undergo a photo chemical reaction to produce a complex organic compounds in the form of Green Algae which is a simple autotrophic organism. By this method the atmospheric carbon dioxide is reduced (carbon sequestration) and also the waste water is treated. This organism contains large amount of triacylglycerol which can be converted by Trans esterification in to biodiesel. The residue which results after the above said process is utilized to produce ethanol, value added chemicals and animal feed stock. This research work was carried out with the financial help of Department of Science and Technology, New Delhi, India. In this work bioproducts of commercial and social importance are to be attempted to be extracted. The algae biomass from the ponds surrounding the Coimbatore city was collected separated and cultivated in the laboratory by utilizing the hostel waste water medium. After initial growth period, calculated biomass were separated and grown in small photo bioreactors with sufficient lighting arrangement. Subsequently this was used for the growth study to find out its feasibility for large scale cultivation. The important factor in the analysis of the biomass is to relate the growth in terms of measurable parameters. Spectroscopic method of analysis was used in determining the biomass growth. The spectroscopic analysis has been substantiated by dry weight measurements. The proposed spectroscopic analysis has been found to be simple and successful in determining the algae growth. This methodology can well be extended to determine the algae biomass in large scale commercial applications especially for algae grown in wastewater and effluent medium. This study will discuss a rate limiting step of the growth of Botryococcus braunii, Chlorella species, Chlorella vulgaris under its optimum growth conditions identified in the lab and the use of kinetic data for large scale cultivation system design and operation.


Keywords : Carbon squestration, Transesterification, Spectroscopic triacyglycerol, Chlorella, Spectrophotimetric analysis