Volume No. 9 Issue No.: 3 Page No.: 547-554 January-March 2015




Kaur Kirandeep, Kaur Arvinder *and Kaur Rajvir

Guru Nanak Dev University, Amritsar, Punjab (INDIA)


Received on : October 10, 2014




A growing interest in environmental genotoxicity has led to the development of several tests for detecting genotoxins in the aquatic environment. One of the most popular and promising test is analysis of micronuclei (MN) in the stressed fish. This test has served as an index of cytogenetic damage over 30 years due to its sensitivity and simplicity. Acute (96h) exposure of azo dye AB113 was given to Channa punctatus under laboratory conditions in semistatic system (with daily renewal of test water) and 96h LC50 was calculated to be 47 mg/l dye. Micronuclei, nuclear anomalies (binuclei, blebbed, notched, lobed, vacuolated and irregular nuclei), cellular anomalies (sphaerocytic, echinocytic, blebbed, notched and vacuolated cells) and necrotic/apoptotic cells were recorded as a measure of cytogenotoxicity of the lethal doses of AB-113 in blood, gill, kidney, liver and spleen of fish. All the abnormalities showed a dose dependent increase and were maximum in the gill. It was observed that analysis of other abnormalities in nuclei and cells in several tissues along with MN yielded better results so their inclusion would help to decide permissible limits of dyes for aquifers in a more accurate way. The findings of this work will also throw light on the possible effects of small doses of the AB113 to human beings on direct (occupational) or indirect exposures (by eating contaminated food) as the results obtained in fish are directly applicable to humans


Keywords : Genotoxicity, Toxicity, Acid Blue-113, Channa punctatus, Micronucleus assay