Volume No. 2 Issue No.: 3 Page No.: 370-377 January-March 2008




Sunita Singh*1, Anita Verma , Amarjeet Bajaj2 and K.N. Singh3

1. Agro Processing Division, Central Institute of Agricultural Engineering, Nabibagh, Berasia Road, Bhopal. (India)


Received on : August 14, 2007




Mango (Mangifera indica L.) kernels are the solid wastes produced in large scale in India when it is processed into pulp and other products. Environmental problem associated with disposal of the kernels rich in starch (50-60% dry basis) is a cause of concern. Attempts have been made as from cassava roots (1) to replace the traditional mechanical method with the use of commercial cell wall degrading enzymes to release starch, in order to obtain high starch yield. This is a newer concept and is not still commercially used in industries. Extraction in this way using microbial enzymes could yield starch from kernel, reported herein this study. Starch was recovered in batch scale using different enzyme concentrations on a two factor Central composite (22CC) Design. To optimize the enzyme concentration to be use for maximum starch yield and calculated regression coefficient.When pectinase was not used as (1) at 9.19 IU and 20.50 U per 10 g dry kernel and (2) at 3.06 IU and 51.25 U per 10 g dry kernel, the starch recovery decreased by 71.3 % and 79.6 % respectively. Similarly if cellulase was not used as in (1) and (2) above, the decrease in starch recovery was not considerable comparatively. The starch yield could be predicted by second order equation with R2=0.92 at P=0.05 asymptotic confidance interval. The kernel also by composition contained more of pectin as compared to cellulose. It was pectinase that was more effective in starch recovery.


Keywords : Mango kernel, Cellulase, Pectinase, Starch, CCD, Eco friendly