Volume No. 11 Issue No.: 2 Page No.: 302-307 October-December 2016
EFFECT OF SUBLETHAL DOSES OF AN AZO DYE, ACID BLUE-113 (AB -113) ON BIOCHEMICAL RESPONSES OF Labeo rohita AND Cirrhinus mrigala
Kaur S., Kaur K. and Kaur A.*
Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab (INDIA)
Received on : April 2, 2016
Present study envisaged to evaluate effect of an azo dye, Acid blue -113 (AB-113) (CI: 26360) on the survival and biochemical changes in the flesh of fry of two important food fishes Labeo rohita and Cirrhinus mrigala. This dye was observed to be very toxic as 96h LC50 came to be 0.54 mg/L for L. rohita and 1.73 mg/L for C. mrigala. Fry became restless and tried to jump out of water. Fins, gills and mouth of the fish absorbed dye in a dose dependent manner. Antioxidant/detoxification enzymes such as glutathione-s-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) were determined in the flesh of L. rohita and C. mrigala after exposure to AB-113 dye. The highest sensitivity was shown by GR in both L. rohita and C. mrigala. The results indicate that the dye is very toxic to the fish and causes significant biochemical changes even at very low doses.
Keywords : Acid blue-113, Antioxidant enzymes, Azo dye, Labeo rohita, Cirrhinus mrigala