Volume No. 11 Issue No.: 2 Page No.: 324-331 October-December 2016

 

BIODEGRADATION OF CYCLOHEXANOL AND CYCLOHEXANONE USING MIXED CULTURE OF PSEUDOMONAS IN ACTIVATED SLUDGE PROCESS

 

Dhanya V*, Usharani M. V.1 and Jayadev P.2

1. Department of Environmental Sciences, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu (INDIA)
2. The Fertilizers and Chemicals Travancore Limited, Cochin, Kerala (INDIA)

 

Received on : September 17, 2016

 

ABSTRACT

 

The petroleum industry is one of the major sources of hazardous waste. Petrochemical wastes are considered to be major sources of hazardous waste resulting in health and environmental risk. The most important constituents include cyclohexanone, cyclohexanol, phenol, cyclohexane, benzene, ketones, carbazols etc. An ecofriendly technique for degradation of such wastes are Bioremediation where microorganisms are exploited to degrade the toxic pollutants to less or non-toxic compounds. The present study was aimed to evaluate the biodegradation potential of selected species of pseudomonas (P. stutzeri, P. alcaligens, P. putida, P. aeruginosa) to degrade cyclohexanol and cyclohexanone by employing activated sludge process. The effect of pH on the degradation of the compounds and percent degradation of the compounds was analyzed using Gas chromatography. Complete biodegradation of cyclohexanone was obtained in the pH 8.5 but in the case of cyclohexanol only 96% of degradation was obtained at the pH 7.2. Mixed consortium of microorganisms has higher biodegradation potential than the single species. This study will contribute a significant part of biodegradation of petrochemicals, which plays a major role in the waste minimization of petrochemical industries for its sustainable development and protect environment.

 

Keywords : Biodegradation, Petroleum hydrocarbon, Cyclohexanol, Cyclohexanone, Activated sludge process

 

 

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