Volume No. 11 Issue No.: 3 Page No.: 511-518 January-March 2017
PRODUCTION OF BIOACTIVE COMPOUNDS FROM MUTANT STRAIN OF Bacillus sp. AGAINST FUNGI
Mahendra K. Gupta*1, Shrivastava A. and Singhal P. K.2
1. School of Studies in Botany, Jiwaji University, Gwalior (INDIA)
2. Department of Biological Sciences, R. D. University, Jabalpur, (INDIA)
Received on : November 10, 2016
The potential of Bacillus species to synthesize a wide variety of antifungal compounds can be used as bio control agent. The increasing prevalence of antibiotics-resistant pathogens and the pharmacological limitations of antibiotics, there is a need for new antimicrobial substances in the form of bioactive compound. Treatment of infectious diseases caused by pathogenic fungal strains was one of the most traditional problems in the clinical field. This necessity encouraged the investigators to synthesize novel and more potent inhibitory bioactive compounds. However, the adverse effects and also appearance of bacterial or fungal resistances influenced the investigators to study on natural products from microorganisms to discover novel and safe bioactive compounds. During present investigation a total of 10 Bacillus sp. were isolated from different environmental conditions. Their antifungal activity was assessed against 5 standard pathogenic fungi viz. Candida albicans (MTCC 1637), Aspergillus niger (MTCC 404), Alternaria solani (MTCC 2101), Curvularia lunata (MTCC 2030) and Fusarium solani (MTCC 3004) by lawn spot and agar well diffusion techniques. Among these the potent bacterial isolate Bacillus lentus showed high antifungal activity. bThe present study is focused on the improvement of Bacillus lentus through random mutagenesis to obtain mutants having enhanced production of bioactive compounds. The Bacillus lentus was subjected for strain improvement by using physical (UV radiation) and chemical (acridine orange) mutation techniques. After the mutation, antifungal activity of the strain was increased in comparison to wild type and different at different time intervals. Extraction of antifungal compounds from both wild and mutant strain was done by using different organic solvents. The molecular weight of extracted antifungal compounds was further determined by SDS-PAGE technique.
Keywords : Bacillus lentus, Bioactive compound, Antifungal activity, Diffusion