Volume No. 11 Issue No.: 4 Page No.: 660-665 April-June 2017

 

PRODUCTION OF CHITOSAN FROM PRAWN SHELL WASTE AND ITS ANTIBACTERIAL ACTIVITY

 

Lambe Pradip, Kale Kishor, Bodre Dilip, Kale Pratibha, Davhare Sonal and Chavan Neha*

Department of Biotechnology, Shivchhatrapati College, Aurangabad, Maharashtra (INDIA)

 

Received on : November 12, 2016

 

ABSTRACT

 

Chitin is the second most abundant natural polysaccharide after cellulose and present in the crustacean exoskeleton, insects and fungi.Chitosan, the deacetylated chitin derivative, is a more useful and interesting bioactive polymer, obtained by the partial deacetylation of chitin which is a natural polymer composed of ?-(1-4) linked D- Glucosamine, randomly distributed.The fish processing industry generates a huge amount of shell waste per processing which usually cause environmental nuisance. Alternatively this waste can be utilized as an economic source of chitin and its derivative chitosan. The present work was undertaken to extract chitin from white sea prawn (Fenneropenaeusindicus ) shell collected from local fish market by combined with chemical method.10% Lactobacillus inoculum prepared in MRS broth was added to the prawn waste along with 5% carbon source (Dextrose) for extraction of chitin. Crude chitin was deacetylated with 50% NaOH to produce chitosan. Chitosan was analyzed for its various physiochemical characteristics such as moisture content, protein content, ash content, solubility and antibacterial activity against various standard bacterial cultures of MTCC and NCIM as well as clinically isolated urine pathogens. The results showed effective antibacterial activity of crude chitosan against all bacterial cultures

 

Keywords : Prawn, Chitosan, Microbial extraction, Characterization, Antibacterial assay

 

 

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