Volume No. 11 Issue No.: 4 Page No.: 693-698 April-June 2017




Shivandappa*1, Agrawal Pushpa2 and Krishna V.3

1. R V Centre for Cognitive Technologies, R V C E Campus, R V Vidyaniketan Post, Mysore road, Bangalore, Karnataka, (INDIA)
2. Department of Biotechnology, RVCE, Mysore road, Bangalore, Karnataka (INDIA)
3. Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, Karnataka (INDIA)


Received on : December 12, 2016




Present supply from oil crops and animal fats account for only approximately 0.3% of the current demand for transport fuels. In contrast, producing biodiesel from biofuel crops is widely regarded as one of the most efficient ways of generating biofuels and also appears to represent the only current renewable source of oil that could meet the global demand for transport fuels. Due to increasing demand for fuel, petroleum fuels diminish at faster rate, as a result of which many efforts have been made for searching new renewable, sustainable energy sources to fulfil the growing demand of transport fuels. There are many alternative energy sources which can be animal or plant source, these source are rich with lipid content. Biomass of these sources consists significant amount of lipid content, which can be used for trans-esterification to yield biodiesel. The yield of the biodiesel is influenced by the Free Fatty Acid(FFA) content of the oil present in biomass of the above sources and amount of FFA in oil indirectly proportional to the yield of biodiesel i.e, as the amount of FFA increases the yield of biodiesel decreases. The modification of the FFA in the lipid content of the biomass can significantly improve the yield of the biodiesel. Keeping these developments in mind, present work concentrated on study of impact of FFA content on yield of Biodiesel by reducing Free FFA in the edible and algal oil. The work begins by modifying FFA content in the oils selected for the production of biodiesel. In order to reduce FFA all the selected oils were subjected to acid treatment prior to chemical trans-esterification. Finally the amount of biodiesel was estimated after trans-esterification of oil. In case of algal oil FFA content around 3.4-4.2% can yield 89-93 ml biodiesel per 100 ml of algal oil. Groundnut oil with FFA content around 2.59-2.66 can yield 82-88ml of biodiesel per 100 ml of groundnut oil. And sunflower oil with 1.1-1.3% can yield 89-92 ml of biodiesel per 100ml of sunflower. Based on these observations we can conclude that as FFA content increases, yield of biodiesel decreases.


Keywords : Botryococcus braunii, Free Fatty Acid, Algal biomass, Trans-esterification, Biodiesel Production