Volume No. 11 Issue No.: 4 Page No.: 699-707 April-June 2017




Parihar Surendra Singh*1 and Pandey Anish C.2

1. Amity Institute of Biotechnology, Amity University Madhya Pradesh, Gwalior (INDIA)
2. Department of Chemistry, Govt. PG College Mungaoli, Madhya Pradesh (INDIA)


Received on : December 12, 2016




The study of bacteriological aspects of water is an important parameter from public health point of view since they play a key role in water borne diseases. Water samples (05 samples for each season and year) collected from Madhav lake of district Shivpuri in Madhya Pradesh, India for pre monsoon and post monsoon during 2009-11 were analyzed for Microbiological parameters. However observation obtained is of 6 years old but still it is very important because status of water quality of said lake is remain unchanged. The MPN (Most Probable Number) value of water in Madhav lake was observed beyond the permissible limit of drinking water quality. Maximum value of Total coliform, fecal coliform and fecal streptococcal coliform was observed 28×105, 18×105, and 15×105 cfu/100ml in post monsoon session. In pre monsoon, the TPC of Madhav lake water ranged between 4.5×104 - 10.5×104 cfu/ml during 2009-2011 and in post monsoon it ranged between 4.1×104 - 19×104 cfu/ml. Total plate count is considered to represent the contamination from the natural environment and also indicate that the presence of bushes and shrubs makes likely possible that, smaller mammals may have been coming around these water bodies to drink water, thereby passing out feces into the water. The profile of the isolated organisms from water samples collected from Madhav lake was analyzed biochemically and found that E. coli, Streptococcus species, E. aerogenes, Pseudomonas species and Salmonella species. The seasonal changes observed in various microbial groups could be related to the influence the physico-chemical properties in the combination with human activities. The bacteriological characteristics of Madhav lake, Shivpuri indicates that its water is highly contaminated and not suitable for drinking.


Keywords : Coli forms, MPN, TPC IMViC, Physico– chemical