Volume No. 12 Issue No.: 3 Page No.: 292-297 January-March 2018




Rabani Mir Sajad*, Gupta Mahendra K.1 and Sharma Richa2

1. School of Studies in Botany, Jiwaji University, Gwalior (INDIA) 2. School of Studies in Microbiology, Jiwaji University, Gwalior (INDIA)


Received on : September 26, 2017




Polycyclic aromatic hydrocarbons (PAH’s) are the ubiquitous organic pollutants found almost in every environment that are released due to incomplete combustion of fossils fuels. These are highly persistent and obstinate contaminants possessing carcinogenic and toxic properties. Thus, their presence in the pernicious concentrations is harmful to the environment. The use of microbes to accelerate the process of degradation of these toxic compounds in soil represents an environment friendly remediation approach besides the other chemical and physical methods. The present research work was carried out to decipher the bacterial abilities from several petroleum contaminated sites for their PAH’s degrading potential. The PAH to be studied was naphthalene, the first member of the PAH’s group and a common micro pollutant in potable water. Enrichment process has been used as a method of choice for the isolation of bacteria using M9 medium supplemented with naphthalene as a sole carbon source. Studies of naphthalene degradation may be significant because naphthalene is a common pollutant that serves as a chemical model for the degradation of PAH’s. In our study 3 bacterial isolates from different soil samples after characterization has shown a good degradation potential. Besides this, heavy metal tolerance of isolates was also determined. The study concludes that isolated bacterial species from contaminated soils can remediate and restore the soil contaminations attributed to other PAH’s and heavy metals also. However, the degradation rate may differ which depends on the source of isolation and carbon source used in the study.


Keywords : Pernicious, Naphthalene, Biodegradation, Petroleum contaminated soil, PAH