Volume No. 12 Issue No.: 4 Page No.: 425-430 April-June 2018

 

ISOLATION AND CHARACTERIZATION OF A NOVEL MUTANT OF Neurosporacrassa HAVING DEFECTS IN BRANCHING

 

Kanojia Paridhi Singh, Vyas Alka and Vyas Harish

1. S. S. in Microbiology,Vikram University, Ujjain, Madhya Pradesh (INDIA)
2. Department of Botany, Govt. Kalidas Girls College, Ujjain, Madhya Pradesh (INDIA)

 

Received on : February, 08, 2018

 

ABSTRACT

 

Neurosporacrassa is a filamentous fungus which is widely being used as a model organism for understanding growth and morphogenesis of fungi. The spherical conidia (macroconidia) of Neurospora germinate and grows in polar manner to form a germ tube which extends by apical growth to form a hypha. The hypha branches subapically to form new hyphal tips which grow and fuse with each other to form a mycelium. We have isolated an interesting mutant of N. crassa by mutagenesis using EMS. This mutant initiates branches at subapical positions but due to some defect some of these branches remain as tiny points on main hypha and are incapable of further growth. As a result these tiny branches appear as beads on the hypha and give it a beaded appearance. In this paper, we have described the characteristics of this mutant and its inheritance pattern.

 

Keywords : Neurosporacrassa, Filamentous fungi, Hypha, Branching, Mutant

 

 

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