Volume No. 12 Issue No.: 4 Page No.: 436-442 April-June 2018

 

ISOLATION AND CHARACTERIZATION OF ARSENITE OXIDIZING STRAINS FOR THEIR POTENTIAL IN ARSENIC BIOREMEDIATION

 

Biswas Rimi and Sarkar Angana

Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha (INDIA)

 

Received on : September 28, 2017

 

ABSTRACT

 

High arsenic groundwater contamination causes serious health risks in many developing countries, particularly in India and Bangladesh. The arsenic fluxes in aquifers are primarily controlled by bacterial populations through biogeochemical cycle. In this present study, two gram positive rod shaped bacteria were isolated from shallow aquifers of Bhojpur district in Bihar, India during the early winter season of 2016, able to withstand arsenite concentration upto70mM and 1000mM of arsenate concentration. They showed high resistance to heavy metals (Ni, Cd, Cu, Cr, Zn, Hg) upto 30mM and utilized some complex sugars along with different carbon sources. Growth at wide range of temperature, pH and salinity were observed. Both these isolates showed high efficiency in converting arsenite into less toxic concentrations of 66.2?M and 46.8?M arsenate respectively from arsenic enriched culture media. Along with superior arsenic transformation and arsenic resistance abilities, the isolates showed a wide variety of metabolic capacity in terms of utilizing a variety of carbon sources under aerobic conditions respectively. This study reports the novel lineages of bacterial populations that can play an important role in subsurface arsenic mobilization and aid in biogeochemical cycling of metallic elements including arsenic.

 

Keywords : Arsenic, Groundwater, Bacteria, Mobilization, Resistance

 

 

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