Volume No. 12 Issue No.: 4 Page No.: 479-482 April-June 2018

 

SACCHARIFICATION POTENTIAL OF Aspergillus flavus ISOLATED FROM SUGARCANE LITTER

 

Pandey Shivangi* and Sharma Tirthesh K.

Department of Botany and Industrial Microbiology, Bipin Bihari College, Jhansi, Uttar Pradesh (INDIA)

 

Received on : September 29, 2017

 

ABSTRACT

 

Leaf litter of sugarcane was collected from the field located in Sonagir of Datia district of Madhya Pradesh, India. Litters from the field were kept in the pits of 2󫎾 feet diameter. After 60 days litter were taken out from the pit and from litter suspension was prepared. 1.0 ml of this suspension was streaked over PDA (potato dextrose agar) medium at pH 6.5. Fungi over culture medium were appeared after 9 days of incubation. Isolated fungi were maintained on Czapek抯 medium and at -20篶 in deep freeze. For saccharification litter was grounded in blender. Powdered litter was used as substrate. Fungi was now optimize for incubation period using 5.0 g/l of substrate and were found that maximum amount of sugar was released on 7.0 days of incubation at found temperature of 35篶 and pH of 6.0. It has also been found that fungi requires 7.3 g/l of substrate along with 4.5 g/l of yeast extract and 3.0 g/l of mannitol.

 

Keywords : Saccharification, Aspergillus flavus, Leaf litter, Optimization

 

 

BACK