Volume No. 13 Issue No.: 1 Page No.: 54-60 July-September 2018




Sharma T. K.* and Singh R.

Department of Botany and Industrial Microbiology, BipinBihari College, Bundelkhand University, Jhansi (INDIA)


Received on : September 29, 2017




Water Hyacinth is used as renewable energy raw material for sugar production and then fuel production because its availability is easy and rate of propagation is very high. It is an aquatic weed, tolerates variation in pH and temperature and is unaffected by toxic substances. Determination of reducing sugar from lignocellulosic biomass of Water Hyacinth was carried out by saccharification process using micro fungi as Aspergillus niger. It was achieved using Dinitros alicyclic Acid (DNS) method. Reducing sugar contents were evaluated maximum at optimum period. The amount of sugar contents were maximum which were 506.0 ?g/ml in untreated condition and 596.0 ?g/ml in treated condition at optimum inoculation period these were found after inoculation of seventh day and 5th days respectively and 398.0 ?g/ml, 545.0 ?g/ml and 420.0 ?g/ml in untreated condition and 665.0 ?g/ml, 645.0 ?g/ml and 567.0 ?g/ml in treated condition at optimum conditions of pH, temperature and substrate concentration which were 6.0 or 5.5 pH, 350C or 300C and 2.5 ml respectively. Here the two types of samples were prepared. One was treated with 1% H2SO4 and other was untreated. The reducing sugar contents were obtained more in treated condition in comparison to untreated condition. The present study evaluated Water Hyacinth as raw material for possible strategies by conversion of hydrolysate to reducing sugar was maximized. For this the effect of optimum condition such as inoculation period, pH, temperature and substrate concentration on conversion of Water Hyacinth biomass for reducing sugar was studied.


Keywords : Aquatic weed, Saccharification, Hydrolysate, optimization, Dinitros alicyclic Acid, Renewable energy