Volume No. 13 Issue No.: 02 Page No.: 108-115 October-December 2018

 

A STUDY ON SERRATIAPEPTIDASE ENZYME PRODUCTION FROM SERRATIA SPECIES

 

Trilokchandran B.*, Agrawal P., Vijayakumar G, Ayush Kumar and Surana S.

Department of Biotechnology, R.V. College of Engineering, R.V. Vidyaniketan Post, Mysore Road, Bangalore, (INDIA)

 

Received on : April 29, 2017

 

ABSTRACT

 

Serratiapeptidaseis an endopeptidase, having molecular weight of about 60 KDa. It absorbs strongly at 275-280nm. Serratiapeptidase is a stronger caseinolytic agent than any other known alkaline or neutral protease. Production of an anti-inflammatory enzyme Serratiapeptidase by fermentation with Serratia species was studied to ascertain optimal nutritional conditions for shake flask production. . The optimized medium contain carbon source, nitrogen source, inorganic nitrogen source, and the optimized fermentation conditions were pH 7.0, temperature 37C and for a duration of 48hr. The fermentation conditions produced a maximum Serratiapeptidase at the end of 24 Hr and the molecular weight of the purified Serratiapeptidase was found to be 51kD.The purification fold was 2.39 after dialysis.

 

Keywords : Serratiapeptidase, Therapeutic, Enzyme, Fermentation, Dialysis, Purification

 

 

BACK