Volume No. 13 Issue No.: 02 Page No.: 169-174 October-December 2018

 

STANDARDIZATION OF ELISA ASSAY FOR DETECTION OF Salmonella enterica

 

Bagul C. D., Bodake N. L.1 and Pethkar A. V.*2

1. Department of Biotechnology, KTHM College, Gangapur Road, Nashik, Maharashtra (INDIA)
2. Department of Microbiology, Govt. Institute of Science, Aurangabad, Maharashtra (INDIA)

 

Received on : February 29, 2018

 

ABSTRACT

 

Salmonella enterica, an organism belonging to family enterobactericeae is responsible for foodborne infection, viz. salmonellosis, enteric fever or typhoid. Monitoring the food samples, raw materials and process equipment for presence or absence of the pathogen is necessary in order to avoid the threat to public health. It is also necessary to develop reliable assays for diagnosis of enteric fever in humans. Conventional detection methods based on physiological and biochemical analyses are time consuming; whereas, newer molecular-based methods are expensive. An indigenously developed immunological ELISA based method could provide a rapid, high sensitivity diagnosis in addition to improved specificity and affordability. Research was undertaken in our laboratory to test the possibility of developing an indirect ELISA assay for infections caused by Salmonella enterica serovar typhi using a heat inactivated strain isolated from pathological sample. Further efforts will be targeted at the direct detection of the pathogen from food samples and food processing equipment.

 

Keywords : Foodborne infection, Pathogen Non lactose fermenting, Typhoid

 

 

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