Volume No. 13 Issue No.: 04 Page No.: 346-353 April-June 2019




Madhuri R. Basutkar and Channappa T. Shivannavar

Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka (INDIA)


Received on : January 12, 2017




With the growing of world’s population, and declined in the available water quantity and its resources. There is a need to search for a permanent solution to conserve and recycle the available water to recycle. For the treatment of waste water(effluents) many advanced techniques are developed like various physical, chemical methods are available, but they were having disadvantages in the proper detoxifying of dyes. These synthetic Reactive azo dyes are highly soluble in water, physicochemical process fails to remove dye completely from waste water. Presently, microbial degradation gave a promising results, in the degradation of dyes. Keeping these aspects, the present investigation was undertaken with an aim of isolation and characterization of potential azo reactive dyes degrading bacterial isolates from different environmental samples, out of those Klebsiella oxytoca CMGS-3 isolated from soil collected from surrounding soil of textile industry, showed maximum decolorisation of Reactive orange-16, with optimized abiotic and biotic factors like pH, temperature, salt concentrations, inoculums concentration, dye concentration, optimization of nutritional sources of energy etc , final decolorisation confirmed by UV-VIS Spectrophotometer.


Keywords : Reactive azo dyes, Effluents, Reactive orange-16, Klebsiella oxytoca CMGS-3, Waste water