Volume No. 13 Issue No.: 04 Page No.: 410-417 April-June 2019

 

STUDIES ON HYDROLYTIC BACTERIA FROM VEGETABLE WASTE, CATTLE DUNG SLURRY AND BIOGAS DIGESTER EFFLUENT

 

V. S. Patil1 and H. V. Deshmukh2

1. Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara, Maharashtra (INDIA)
2. Department of Microbiology, Yashawantrao Chavan Institute of Science, Satara, Maharashtra (INDIA)

 

Received on : December 29, 2018

 

ABSTRACT

 

Biomethanation is the anaerobic digestion of biodegradable organic matter in an enclosed space under controlled operational conditions to produce biogas and manure. Biomethanation is considered as eco-friendly microbiological treatment method for management of different kinds of agro-industrial wastes. The types of microorganisms involved in biogas production process are mainly bacteria, yeast, molds and actinomycetes. Hydrolytic microorganisms play an important role in initial step of biomethanation. The present paper deals with the study of bacteria from vegetable waste slurry, cattle dung slurry and biogas digester effluent. The biomethanation experiment was carried out in 5 litre capacity digester under ambient temperature conditions. Standard plate count of bacteria from vegetable waste slurry, cattle dung slurry and digester effluents were found to be 3.27 x 107, 2.44 x 109 and 2.25 x 1011 CFU/mL respectively. In present study sixteen, twelve and seventeen bacteria were isolated and identified from vegetable waste slurry, cattle dung slurry and digester effluent respectively using standard methods. Further all the bacterial isolates were tested for their potential to produce hydrolytic enzymes. The present study has provides useful information about the bacteria associated with substrates and digester effluent that have important role in biogas generation. Further, the potent hydrolytic bacterial isolates can be used to develop consortia to increase the efficiency of vegetable waste and other agro-industrial organic wastes based biomethanation process and also for its composting.

 

Keywords : Vegetable waste, Environmental pollution, Biomethanation, Bacteria, Hydrolytic enzymes

 

 

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